Contact an Account ManagerComparison of the separation of β-lactam antibiotics on NUCLEOSHELL RP 18 and on NUCLEODUR® C18 Gravity
Summary
Type: HPLC
Application #: 124940
Catalog#: 763132.40 - MN HPLC Column, Nucleoshell RP 18, 90A, 2.7um, 4x50mm
Detection:
Conditions:
NUCLEOSHELL RP 18 NUCLEODUR® C18 Gravity
Eluent A: acetonitrile acetonitrile
Eluent B: 20 mmol/L KH2PO4, pH 3.5 20 mmol/L KH2PO4, pH 3.5
Gradient: 10 % A for 0.5 min,
10–50 % A in 1.5 min,
hold for 0.5 min 10 % A for 3.0 min,
10–50 % A in 9.0 min,
hold for 3.0 min
Flow: 2.0 mL/min 1.0 mL/min
Temperature: 25 °C 25 °C
Injection volume: 1.0 µL 1.0 µL
Back pressure : 270 bar 110 bar
Authors: HPLC department
Source: Macherey-Nagel, Germany 2011